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  Indian J Med Microbiol
 

Figure 8: Treatment with siRNA targeted against UCP3 resulted in metabolic changes in vitro. A, UCP3 siRNA groups had decreased 5' AMPK and caspase 3 expression levels. The expression of PGC-1a was not affected solely by UCP3 siRNA. B, RT-PCR revealed changes to key enzymes involved in fatty acid oxidation, showing decreased ACOT2 and PPARμ mRNA levels, with unchanged ACOT1 and CPT1-α mRNA levels. *P < 0.05, **P < 0.01,***P<0.001 vs. the sham group; #P < 0.05, ##P < 0.01, ###P < 0.001 vs. the TAC group. Results are expressed as the mean ± SD. UCP3: Uncoupling protein 3, AMPK: AMP-activated protein kinase, PGC-1α: Proliferator-activated receptor-gamma coactivator-1α, ACOT2: Acyl-CoA thioesterase 2, PPARβ: Proliferator-activated receptor β, CPT1-α: Carnitine palmitoyltransferase-1α, SD: Standard deviation

Figure 8: <b>Treatment with siRNA targeted against UCP3 resulted in metabolic changes <i>in vitro</i>.</b>
<b>A</b>, UCP3 siRNA groups had decreased 5' AMPK and caspase 3 expression levels. The expression of PGC-1a was not affected solely by UCP3 siRNA. <b>B</b>,  RT-PCR revealed changes to key enzymes involved in fatty acid oxidation, showing decreased ACOT2 and PPARμ mRNA levels, with unchanged ACOT1 and CPT1-α mRNA levels.
*<i>P</i> < 0.05, **<i>P</i> < 0.01,***P<0.001 vs. the sham group; #<i>P</i> < 0.05, ##<i>P</i> < 0.01, ###<i>P</i> < 0.001 vs. the TAC group. Results are expressed as the mean ± SD. UCP3: Uncoupling protein 3, AMPK: AMP-activated protein kinase, PGC-1α: Proliferator-activated receptor-gamma coactivator-1α, ACOT2: Acyl-CoA thioesterase 2, PPARβ: Proliferator-activated receptor β, CPT1-α: Carnitine palmitoyltransferase-1α, SD: Standard deviation